RP-HPLC法检测0.2%苯酚溶液含量

杨敏，刘信奎，刘峰等.RP-HPLC法检测0.2%苯酚溶液含量[J].黑龙江医药,2012,25(2).189-190

苯酚;高效液相色谱;含量测定

杨　敏，刘信奎，刘峰，张欣愉，孙伟，宋刚，贾首时

齐齐哈尔市食品药品检验所(齐齐哈尔　161006)

2012年

189-190

知网

目的：建立HPLC法测定苯酚溶液的含量。方法：色谱柱为Waters-C18(150mm×4.6mm,5μm)，流动相为甲醇-水(65：35)，检测波长254nm，流速1.0ml?min-1，进样量10μl。结果：苯酚溶液进样量在2.021μg～20.21μg?ml-1范围内线性关系良好(r=0.9996，n=5)平均回收率99.7%，RSD=0.81(n=9)。结论：本方法简便，准确，重复性好，可用于该制剂的含量测定。 To establish a method of determination of Phenol solution by RP-HPLC.Methods：RP-HPLC was used Waters-C18(150mm×4.6mm,5μm)column， mobile phase was composed of methanol-water (65：35)， The flow rate was 1.0ml?min-1，the UV detection wavelength was 254nm. Results： The linear range of Phneytoin Sodium was 2.021μg～20.21μg?ml-1 (r=0.9996，n=5). The average recovery was 99.7% and RSD was 0.81(n=9). Conclusion： The method is simple， accurate and suitable.。