Caspase-8在慢性肝炎患者肝组织中的表达及意义

半胱氨酸天冬氨酸蛋白酶8 肝炎,慢性 细胞凋亡

半胱氨酸天冬氨酸蛋白酶8 肝炎,慢性 细胞凋亡

杨海峰，薛玲，苗益辉，郑彩娟，幺作义，张艳淑，姚林

(河北联合大学公共卫生学院,河北唐山,063000)

2013年

138-141

知网,万方

目的 探讨Caspase-8在慢性乙型肝炎、慢性丙型肝炎和非酒精性脂肪性肝炎中的作用及机制.方法 应用免疫组化方法对70例慢性乙型肝炎、50例慢性丙型肝炎、50例非酒精性脂肪性肝炎和15例正常肝组织进行Caspase-8检测.结果 Caspase-8在慢性乙型肝炎、慢性丙型肝炎、非酒精性脂肪性肝炎中的表达(58.6％、64.0％、82.0％)高于其在正常肝组织中表达(13.3％),差异有统计学意义(P＜0.05)；并与肝细胞凋亡密切相关(r=0.409,P＜0.001).Caspase-8在重型肝炎中的表达阳性率高,轻型肝炎表达阳性率最低,差异有统计学意义(P＜0.05).Caspase-8在不同ALT水平肝炎患者肝组织中的表达差异无统计学意义(P＞0.05)；结论 Caspase-8活性增强导致细胞凋亡增多,在肝病理生理过程中起着重要作用. Abstract:Objective To investigate the roles and mechanisms of the apoptosis effector molecule hepatitis B(CHB)，chronic hepatitis C(CHC)，and non一alcoholic steatohepatitis(NASH). caspase一8 in the pathogeneses of chronic Methods Caspase一8 protein expression was examined by immunohistochemistry in liver biopsy samples from 70 patients with CHB，50 patients with CHC and 15 healthy controls. assay. The significance Histological analysis was performed to grade extent of inflammation and fibrosis. Apoptosis was atients with NASH, detected by TL?NEL of intergroup differences were assessed by Chi一squared test or Fisher' s exact test Spearman’s rank correlation coefficient NASH(82.0 %)liver tissues than in closely related to liver cell apoptosis(r tent of pathological injury(P>0. OS). Results Caspase一8 expression was significantly higher in CHB ，and correlation was assessed by (58.6%)，CHC(64.0%)，and healthy control liver tissues(13.3%)(all P。