唐山地区献血人群隐匿性乙型肝炎病毒感染分析

曹晓，曹庆宝，张国强等.唐山地区献血人群隐匿性乙型肝炎病毒感染分析[J].临床肝胆病杂志, 2013, 29(2).117-119

肝炎,乙型； 肝炎病毒,乙型； 酶联免疫吸附测定；

曹晓; 曹庆宝; 张国强; 常在娟; 李淑萍; 徐微微

(唐山市中心血站,河北唐山,063000)

2013年

117-119

知网,万方

目的了解唐山地区无偿献血人群隐匿性乙型肝炎感染情况。方法用ELISA法检测无偿献血者的乙型肝炎血清标志物,对于HBsAg阴性样本,进行HBV核酸检测(NAT),NAT阳性样本,用罗氏试剂确证HBV DNA载量。结果共检测116 741例血样,证实隐匿性乙型肝炎感染者35例,占总献血人数的0.29‰。其中97.1%隐匿性乙型肝炎感染者样本的HBV DNA滴度低于102IU/ml。在HBV DNA阳性人群中,抗-HBc阳性率较高,占81.5%,抗-HBs阳性或乙型肝炎病毒血清标志物全阴性也可检出HBV DNA分别占55.6%和22.9%。结论唐山地区献血人群中血清HBsAg阴性者存在一定比例的隐匿性HBV感染,其HBV病毒载量均较低,核酸检测能够提高HBV感染的检出率。 To investigate tlle pKvalence of occult hepatitis B vims(HBV)infel tion(OBI)among the volullteer blood donors in Tangshan, China, RIetllods Enzyme-linked in1munosorbent assay was uscd to detect scrum HBⅤ markcrs in thc blood donors NucIeic acid test(NAT)was pelfomed in the hepatitis B sulface antigen(HBsAg)-negative blood samples to detect HBⅤ DNA,and Roche rea- gent was uscd to measure thc viral load of HBⅤ DNA in NAT-positlve samples. Results Among the HBsAgˉ negative blood samples of 116741blood donors,39(0033%)had positive resullq in NAT,and35 (0.029%) wcrc confirmed With OBI lhe vllal load of HBⅤ DNA was less than102IU/ml in97 1%ofthc blood donoⅡ with OBI,Ofthc blood donom with OBI,27(77.1%)were posithe for at least one of hepalltls B sulface anⅠ body(HBsAb),hepatltis B e anhgen,hepatltls B e antibody,and hepati‘ s B core antibody(HBcAb),and 8(22.9%)w∝ e negathe for a11∞ mm HBⅤ malkers ofthe27positivc blood donclrs,22(81.5%)wcrc positive for HBcAb,and15 (55.6%)were positlve for HBsAb Conclusion obi occurs in some HBsAgˉ negathe blood donorq in Tangshan,and the viral load of HBⅤ DNA is1ow NAT is effective in increa“ ng the detection rate of HBⅤ infcction。